RPMI 1640 Medium - Modification 12RPMI 1640 Medium Modification 12 contains L glutamine and 25 mM HEPES, but does not contain sodium bicarbonate and is a versatile and essential medium widely utilized in animal cell culture, particularly for the growth and maintenance of various cell types. This enriched formulation is designed to provide an optimal environment that supports cellular metabolism, proliferation, and function. The addition of L glutamine serves as a vital amino acid that