10X Tris-HEPES SDS Running Buffer, pH 8.0, 1L" Specification Value Concentration 20X Name Tris HEPES SDS Running Buffer pH at 1x 8. 0 Volume 1L SKU THS 10 0100 Composition at 1x Tris 0. 1 M, HEPES 0. 1 M, SDS 3 mM A running buffer optimized for Tris HEPES polyacrylamide gels, providing enhanced resolution of mid range molecular weight proteins by SDS PAGE. This formulation delivers fast run times and sharp band separation, improving laboratory throughput. The buffer system is particularly